Dilution factor

Question

Your supervisor provides you with a 1.0 mg/mL solution of Bovine Gamma Globulin (BGG). What is the dilution factor if you add 150 µL of 1.0 mg/mL BGG to 150 µL of diluent?

Answer 1

This is a C1 * V1 = C2 * V2 problem

C1 is the initial concentration C2 is the final concentration

V1 is the initial volume V2 is the final volume

Dilution Factor is the factor by which the stock solution is diluted.

It may be expressed as the ratio of the volume of the final diluted solution (V₂) to

the initial volume removed from the stock solution (V₁), as shown in the equation above.

Dilution factor may also be expressed as the ratio of the concentration of stock solution (C₁) to

the concentration of the diluted solution (C₂).

dilution factor = V2/V1= C1/C2

V1 = 150μL of BGG solution

V2 = V1 + 150μL of diluent

set up V2 divided V1 and calculate the dilution factor