A researcher is studying the effects of bleach on blue food dye, C37H34N2O9S3Na2 (792.86 g/mol).
(a) Water is added to 0.200 g of the dye until the volume is 500. mL. What is the molarity of the solution? Show your calculation.
(b) The chemist uses a 0.50 mL sample of the solution prepared in (a) to prepare a 100.0 mL diluted solution. What is the concentration of the diluted solution?
The diluted solution is placed in a dry 3.0 cm cuvette, and its absorbance is measured using a spectrophotometer set to a wavelength of 412 nm. A calibration curve for this dye is shown below. Note the concentration is given in micromolar on calibration curve. (1 M = 1 x 106 µM)

(c) Determine the molar absorptivity of the dye.
(d) What would be the measured absorbance if the concentration of the dye was changed to 1.04 x 10-5 M?
J.R. S.
11/07/20