- The molecule has a bond that will change energy level by an energy amount equal to that in photon at the correct wavelength. The shorter the wavelength the higher the photon's energy.
- If the molecule has only one quantum jump in an energy that is in the visible light range, That color of light is the only one that will interact with the dye.
Jose C.
asked 11/01/19Need help answering this questions as soon as possible.
1: What happens in a molecular level that allows an analyze to absorb light at a certain wavelength.
2: Why are you using a specific wavelength of light to record absorbance measurements for the dye (i.e., why non't every wavelength of light be absorbed by the analyte)
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