J.R. S. answered • 06/10/18
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Convert to get all units the same.
25 mg/ml x 1000 ug/mg = 25,000 ug/ml Stock solution
250 ug/45 ml requires one to obtain 250 ug from the stock: 25,000 ug/ml (x ml) = 250 ug and x = 0.01 ml = 10 ul
Thus, Hannah would need to take 0.01 ml or 10 microliters from the stock, and then dilute that to a final volume of 45 ml.
J.R. S.
tutor
Lilly,
- I used ug/mg as the conversion factor, i.e. 1000 ug/mg. So, when you multiply 25mg/ml x 1000 ug/mg, the mg cancel and you’re left with ug/ml.
- Usually you would just add 10 ul to 45 ml.
- Yes, you certainly may use V1C1 = V2C2 as long as you keep all units consistent
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06/10/18
Lilly A.
Thank you very much indeed for the reply Prof. J.R.S. ! This has helped me immensely !
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06/10/18
Lilly A.
- I understand to convert 25 mg/ml x 1000 ug/mg = 25,000 ug/ml Stock solution, but why ug/mg did you mean ug/ml instead?
- I understand to add 10 microliters from the stock to 45ml. As this is a very small amount. Usually would you add 10microliters to 44,990 microliters to make up the new solution ?
- Can you use the V1C1=V2C2 formula :
V1: unknown06/10/18