Sonia B.

asked • 10/12/17

volume of PCR , is this right?

Reagent stock concentration             Required final concentration                Volume
10X Buffer                                                       1X                                       5 ul
25 mM MgCl2                                                 2 mM                                     4
10 mg/mL                                                 BSA 0.3 mg/mL                            1.5
10 mM dNTPs                                                0.2 mM                                     1
10 μM F primer                                              0.2 μM                                      1
10 μM R primer                                             0.2 μM                                       1
Taq DNA polymerase (12,500 U/mL)             50 U/mL                                       0.2
5 μg/μL Template DNA                               0.2 μg/μL                                        2
Water                                                  Make up to final volume                       34.3
Final Volume                                                                                             50 μl
Is this right I calculated the figures in BOLD using c1v1=c2v2

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