template and coding DNA

Question

If the template strand of DNA is 3’-xxx-5’, what are the first 4 amino acids of the protein?
If the coding strand of DNA is 3’-xxx-5’, what are the first 4 amino acids of the protein?

Template:                               Coding:                        Answers
TAC.AAA.TTG.CCA              TGG.CAA.TTT.GTA
TAC.TAA.CGG.GCA             TGC.CCG.TTA.GTA
TAC.TAT.GTA.CAC               GTG.TAC.ATA.GTA

Answer 1

I'm not sure the 2nd question is intended as a trick; the intent may be to think of the strand as being written in reverse order. In other words, the while the default is

5'-TGG.CAA.TTT.GTA-3'

 

the second question is asking about

3'-TGG.CAA.TTT.GTA-5'

 

If this is the correct interpretation, you should rearrange to the normal polarity (simply re-write from right to left) to get:

5'-ATG-TTT-AAC-GGT-3'

 

Once re-arranged, you would use a codon table to generate the correct amino acid sequence.

AUG = methionine (the famous 'start' codon)[remember that in RNA, uracil is used instead of thymine, so you should look up AUG instead of ATG, UUU instead of TTT, etc.)

Answer 2

Second part of the question appears to be a trick, coding strand is never  3’-xxx-5’

 

TGG.CAA.TTT.GTA Trp, Gln, Phe, Val,

  TGC.CCG.TTA.GTA Cys, Pro, Leu, Val

GTG.TAC.ATA.GTA Val, Tyr, ile, Val

 

Best