diluting different antigens

Question

I have 6 different antigens each at 40,000 PNU/cc. I want to get a combined dose of 10,000 PNu/cc in a 10 cc vial. how would i dilute this? What formula?

Answer 1

Hi Davies,

You'll want to use the dilution formula (C₁V₁=C₂V₂) to solve this problem, where C is the concentration and V is the volume. We'll be solving for V₁, given that we know all the other variables.

(40,000)x=(10,000)(10)

x=100,000/40,000

x=2.5cc

 

So this means that you need 2.5cc of each antigen in the vial. Unfortunately, this means that if you do the dilution, you'll end up with 15cc instead of 10cc [because (2.5cc)(6)=15cc]. 

 

Are you sure you wrote the problem correctly for us?