Few media in my micro lab is just expired (manufacturer specification). Can i reuse it after validation? is there any method for such validation.

Question

Expiry dates: may 2015

Answer 1

Hi Rekha,

 

If the media is for standard bacterial culture and in unopened bottles then they should be fine. However, I would not trust the bottles that were opened or the liquid is cloudy or with a precipitation at the bottom of the bottle.

 

If you wish and have time then you may grow small o/n culture of 5 ml with a bacterial colony in a falcon 2059 tube; at the same time make a negative control of another 5 ml of the same medium with no colony. If negative tube is clear after o/n incubation then it is indicative that your media is fine.

 

Hope this helps.

 

Naina B. PhD