Tysir S. answered • 12/23/22
"Expert in chemical engineering with advanced training"
It is generally recommended to produce your own BSA curve for the protein content analysis of an unknown sample, as this will allow you to ensure the accuracy and reliability of your results. This is because the characteristics and properties of different BSA samples may vary, and using an online BSA curve or a calibration curve from a different source may not accurately reflect the characteristics of your sample.
To produce your own BSA curve, you will need to prepare a series of BSA standard solutions at different concentrations, and measure the absorbance of each standard solution at the wavelength of interest using a spectrophotometer. You can then plot the absorbance values of the standard solutions against their concentrations to generate a calibration curve. Once you have your own BSA curve, you can use it to determine the protein concentration of your unknown sample by measuring its absorbance and interpolating the absorbance value on the calibration curve.
That being said, if you are unable to produce your own BSA curve for any reason, you may still be able to use an online BSA curve or a calibration curve from a different source as a rough estimate of the protein concentration of your sample. However, it is important to keep in mind that the accuracy and reliability of your results may be compromised when using an online BSA curve or a calibration curve from a different source, and you should use caution when interpreting your results.
