It has been hypothesized that vitamin D is associated with androgen levels in men. We,
therefore, aimed to evaluate whether vitamin D supplementation increases serum total
testosterone (TT) levels in men with low TT levels at baseline. Methods: The Graz Vitamin
D&TT-RCT is a single-center, double-blind, randomized placebo-controlled trial conducted
between March 2013 and November 2017 at the endocrine outpatient clinic at the Medical
University of Graz, Austria. One-hundred healthy men with serum TT levels < 10.4 nmol/l and
25-hydroxyvitamin D [25(OH)D] levels < 75 nmol/l participated in the trial. Subjects were
randomized to receive 20,000 IU of vitamin D3/week (n = 50) or placebo (n = 50) for 12 weeks.
Primary outcome was TT measured using mass spectrometry. Secondary outcomes were free
testosterone, free androgen index, sex hormone-binding globulin, estradiol, follicle-stimulating
hormone, luteinizing hormone, metabolic characteristics, and body composition. Results:
Ninety-four men [mean age and 25(OH)D: 47 (± 12) years and 56.3 (± 18.3) nmol/l, respectively]
completed the study. We found no significant treatment effect on serum TT or on the remaining
secondary outcome variables. Conclusion: Vitamin D treatment had no effect on serum TT
levels in middle-aged healthy men with low TT levels.
Independent variable:
Dependent variable __________________________________
List a maximum of 3:
A. Nominal variable: ________________________________________
B. Ordinal: ________________________________________
C. Interval: ________________________________________
D. Ratio: ________________________________________