Organic Chemistry College Homework Help Please

Question

DNA template strand: 3’ GGC ACT ATT GCT 5’

a)   Write corresponding mRNA segment.

b)   What are the anticodons of the tRNA?

c)   Write the 3 letter abb. for the sequence.

thank you

Answer 1

Hi Gabrielle,

When writing the corresponding mRNA strand, we must consider (a) directionality and (b) the nucleotides bases in RNA. Transcription occurs when RNA Pol. reads the DNA template strand from 3' → 5', synthesizing mRNA from 5' → 3'. RNA also utilizes uracil (U) instead of thymine (T).

When considering complementary binding between mRNA to tRNA, we must also consider directionality and the appropriate nucleotides. As another form of RNA, tRNA also uses uracil instead of thymine. Similarly, tRNA also reads mRNA from 5' → 3', so anticodons must bind complementarily from 3' → 5'.

Keep in mind, when writing the peptide sequence, the code is written for the 5' → 3' mRNA strand. Interestingly, it seems like your professor gave you a DNA template strand that codes for two STOP codons.

Given the DNA Template: 3’ GGC ACT ATT GCT 5’

mRNA Strand: 5' CCG UGA UAA CGA 3'

tRNA anticodons: 3' GGC ACU AUU GCU 5'

Protein Sequence: Pro Stop Stop Arg