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How can you estimate the mean and standard deviation if your given two percentiles and their values

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The easiest way to answer this question is to set up a system of equations using the definition of the z-score. The z-score is defined as:

z = (x - μ)/σ

Where is the value, μ is the mean, and σ is the standard diviation.  For a normal distribution, the precentile can be calculated from the z-score.  These value of the percentile for each z-score can be found in various tables (see:  For the 15.9th percentile, the z-score is -1.0 and for the 97.7th precentile, the z-score is +2.0.  Thus, we have the following system of equations:

-1.0 = (25 - μ)/σ   =>   μ - σ = 25

+2.0 = (40 - μ)/σ   =>   μ + 2σ = 40

Solving this system of equations, one finds the mean to be 30 and the standard diviation to be 5.