10/21/23

BIOCHEMISTRY-DILUTE LDH?

In biochemistry lab, why do you have to dilute the LDH before adding to the assay? (we used E.Coli, and imidazole in lab, I don't know if that is important)

10/20/23

What does phospholipase C do?

What does phospholipase C do?it activates RAF, a protein kinase, which initiates a phosphorylation cascadeit cleaves phospholipids into IP3 and DAG, which are both second messengersit creates cAMP,... more

10/20/23

What does "heterotrimer" mean?

a receptor that has two subunits that phosphorylate each othera protein that activates another molecule by phosphorylating ita protein that has three subunits, all the same polypeptidea protein... more

10/20/23

In signal transduction diagrams, how do we depict "A inhibits B?"

A ------ BA xxxxxx BA x BA ----> BA ----| B

10/20/23

I have a report due tomorrow and I am lost:( please help!

How do you estimate the %B responsible for eluting LDH from the column?

10/20/23

Chromatograms-having trouble on my lab report, please help:)

How do you identify the peak fraction for flowthrough (FT) on a chromatogram and identify elution peaks seen in the chromatogram. How do you identify the LDH peak and how do you know it is the LDH... more

10/17/23

Consider the structure shown.

Determine the number of shared and unshared electrons in the structure.shared (bonding) electrons:unshared (lone, nonbonding) electrons:Determine the number of shared and unshared pairs of... more

10/16/23

Please Explain Oxidative Phosphorylation?

Hi I'm a college student studying microbiology. Right now I'm studying the way animal cells make ATP through the Mitochondrion. there are three things that have to take place for ATP to be created... more

10/16/23

Percent Yield Question

When copper is heated with an excess of sulfur, copper(I) sulfide is formed. In a given experiment, 0.0970 moles of copper was heated with excess sulfur to yield 3.77 g copper(I) sulfide. What is... more

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