2 Answered Questions for the topic fluorescent microscopy
07/27/19
How does formaldehyde/PBS or methanol fixation of cells affect lysosomal pH?
**The question** is fairly simple - does formaldehyde or methanol fixation in preparation for immunocytochemistry/immunofluorescent staining affect the pH of the lysosomes?**Some background:** I'm...
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06/24/19
How to convince suspension cells to adhere more tightly?
I'm developing a cell-based assay in 96-well plates that requires adherent cells, as they need to be washed at least twice during the protocol. I'm using in-house strains of...
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