Louis I. answered 04/28/19
Computer Science Instructor/Tutor: Real World and Academia Experienced
My understanding is, "no" for most modern distros, although the frequency of crontab file(s) update-check may vary, updates should be reloaded by the cron service "in short order".
If you want to be absolutely sure (and for good luck), you can do something like this:
sudo service cron reload
... assuming you have admin/sudo rights.