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Sample Microbiology Review Sheet

Brucella abortus
• GN cocco-bacilli
• Brucellosis
• Infected animal or aerosols
• Abortions in cattle
Rickettsias
• Non-motile, GN, non-spore forming, can present as cocci, rods or thread-like, pleomorphic
• Reproduce only inside a living cell, parasitic in fleas, ticks, lice, and mites and transmitted by bite to vertebrate hosts, including humans,
• Typhus and Rocky Mountain spotted fever.
• Include the genera Rickettsiae, Ehrlichia, Orientia, and Coxiella
Caulobacter
• Caulobacter crescentus
• GNR
• Oligotrophic bacterium widely distributed in fresh water lakes and streams.
• Caulobacter is an important model organism for studying the regulation of the cell cycle, asymmetric cell division, and cellular differentiation.
Rhizobium
• Soil bacteria that fix nitrogen (diazotrophs) after becoming established inside root nodules of legumes (Fabaceae).
• Rhizobia require a plant host
• GNR, motile, non-sporulating rods.
Neisseria gonorrhoeae
• Gonococci, coffee bean-shaped diplococci
• Fastidious GN cocci that require nutrient supplementation to grow in laboratory cultures. Grow on chocolate agar with carbon dioxide.
• Thayer-Martin
• NAAT or PCR, see chlamydia
Bordetella pertussis
• GN, aerobic coccobacillus capsulate
• Causative agent of pertussis or whooping cough.
• Unlike B. bronchiseptica, B. pertussis is not motile.
• growth on Bordet-Gengou agar or BCYE plate with added cephalosporin to select for the organism, which shows mercury-drop-like colonies
Thiobacillus
• Acidithiobacillus is the most important genus of chemolithotrophs that metabolize sulfur.
• It includes motile rod-shaped cells that can be isolated from rivers, canals, acidified sulfate soils, mine drainage effluents, and other mining areas.
• Biobleaching whereby metals are extracted from their ores through oxidation.
• The bacteria are used as catalysts.
Pseudomonas aeruginosa
• GNR
• Oxidase, citrate & catalase positive
• Non-fermenter,
• Pyocyanin, blue green pigment
• Grapelike odor
• The oxidative-fermentative test determines if certain gram-negative rods metabolize glucose by fermentation or aerobic respiration (oxidatively)

$60p/h

Risa T.

Microbiology & Molecular Biology Specialist

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