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DNA topoisomerase II unwinds DNA using the free energy derived from ATP hydrolysis to ADP and Pi. To follow continuously the catalytic turnover of DNA topoisomerse II, an enzymatic assay needs to...

Staphylococcal nuclease accelerates phosphodiester bond hydrolysis by a factor of 5.6x1014 over the spontaneous rate (1.7x10-13). How much does staphylococcal nuclease decrease the activation energy...

Staphylococcal nuclease accelerates phosphodiester bond hydrolysis by a factor of 5.6x1014 over the spontaneous rate (1.7x10-13). How much does staphylococcal nuclease decrease the activation energy...

The covalent catalysis mechanism of an enzyme depends on a single active site Cys whose pKa is 8. A mutation in a nearby residue alters the microenvironment so that this pK increases to 10. Would...

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