Hello, I am working through a little case study exercise related to insulin resistance, which is the primary cause of type-2 diabetes. According to my reading, "a continuous over stimulation of insulin signaling alters how the insulin receptor and its down-stream signaling pathways will respond to insulin."
Here is a list of possible research topics to investigate and determine their relative role in insulin resistance:
Some of the documented changes in insulin signaling as a result of diabetes are:
1. Decreased insulin receptor kinase activity upon binding of insulin to the receptor.
2. Decreased binding of signaling effectors to the insulin receptor due to: a) less phosphorylation sites on the insulin receptor, and b)feedback inhibition on the signaling molecule, preventing its binding to the insulin receptor.
3. Decreased signaling effector binding leads to defective downstream activation of the kinase cascade and second messenger signaling pathways.
4. Decreased glut4 (glucose transporter) fusion to the cell membrane and less glucose transported into the cell.
Considering these topics, which project would you pick to study and why? What kind of experiments would you do?
I am really lost on this, and it looks like any potential topic could be a good one, but I was just wondering on your reasoning between picking one project over the other and how you would experiment it.
Thank you for any help! I am really lost!