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James T.

Austin, TX

$42/hour

Biotechnology/Science Tutor and full-time Medical Technologist

In-person lessons

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Austin Community College
Biotechnology

Education

Austin Community College (Biotechnology)

About James

I am a medical technologist with the Texas Department of State Health Services in the Biochemistry and Genetics department of the Newborn Screening lab. I recently received my Associates of Applied Science, in Biotechnolgy, from Austin Community College (GPA 3.69). Prior to ACC I attended Texas A&M University in Galveston, with a focus on Marine Biology. The ACC Biotech program provided me with the most current methods, techniques, and concepts used in the biotechnology industry and research and development labs throughout the world.

In my current position I perform automated Fluorometric ELISA assays to detect 17-OHP, IRT, TSH, AND T4 in neonatal patients.

I've worked as a contracted Microbiologist for the TX DSHS Diagnostic Serology and HIV/STD labs, as well as functioning as a lab tech III in the Newborn Screening Specimen Receiving Lab. I worked the graveyard shift at CPL as a lab assistant while interning at Bioo Scientific in the Food and Feed Department. During my internship at Bioo Scientific I performed QC on ELISA test kits and developed 2 new ELISA kits for the company.

I have performed the extraction, purification, and isolation of various recombinant proteins and nucleic acids (plasmid DNA, genomic DNA and RNA) using freeze-thaw, sonication, mechanical, bead, and chemically mediated cell lysis. I have experience in amplification of nucleic acid sequences using end-point PCR, Real Time PCR, and reverse transcriptase PCR. I have also performed agarose Gel Electrophoresis and Pulse Field Gel Electrophoresis to analyze nucleic acid samples.I am experienced in chromatography purification techniques including hydrophobic interaction, size-exclusion, ionic, gravity, affinity, FPLC, HPLC, and GC. I'm very comfortable with protein analysis using SDS-PAGE, Western Blot, ELISA, and enzyme assays (Bradford). My "Molecular Techniques" class published 3 novel DNA sequences for the GAPC gene of three novel plant species. I enjoy using bioinformatics (NCBI, CN3D, BLAST,etc). While attending ACC, I was awarded a National Science Foundation scholarship and participated in an associated biofuel research project with the Department of Defense (2011-2013). Our research project focused on the production, purification, and isolation of biofuels and biomass using recombinant algae and the isolation of potentially profitable components. The project was a success and the proprietary information was purchased by a private company in 2013.

This summer I participated in an internship at the Texas Department of State Health Services Molecular Biology Lab. The lab is responsible for the identification of infectious diseases as well as monitoring and responding to outbreaks for the entire southwest United States. During my time with in the molecular biology lab, I performed conventional and molecular serotyping of infectious diseases and validation and development of an intestinal parasite screen using real time multiplexed PCR. My final project in the state lab dealt with a relatively new technology that uses loop-mediate isothermal reactions to amplify nucleic acids in about an hour without expensive visualization equipment.

I was a United States Swim Coach for almost 13 years. In my time coaching I've adopted a teaching style that uses positive reinforcement and encourages discussion. I hope I can help you meet your goals this year.
I am a medical technologist with the Texas Department of State Health Services in the Biochemistry and Genetics department of the Newborn Screening lab. I recently received my Associates of Applied Read more

Policies
Cancellation
2 hours notice required
Travel Radius
Travels within 25 miles of Austin, TX 78754
In-person lessons
Science:
ACT Science, Biology,
Chemistry, Genetics,
Microbiology,
Physical Science
Test Preparation:
ACT Science

Approved subjects are in bold.

Approved subjects

In most cases, tutors gain approval in a subject by passing a proficiency exam. For some subject areas, like music and art, tutors submit written requests to demonstrate their proficiency to potential students. If a tutor is interested but not yet approved in a subject, the subject will appear in non-bold font. Tutors need to be approved in a subject prior to beginning lessons.

Biology

I have an Associate of Applied Science in Biotechnology. I feel confident in my understanding of Biology from General Bio to Molecular Biology. I understand the importance of the Central Dogma of Biology "DNA>RNA>Protein". I was awarded the National Science Foundation S-STEM scholarship and participated in an associated Department of Defense research project to extract,purify, and isolate biofuels from recombinant algae.

Genetics

I am skilled in the techniques of extracting, purifying, and analyzing nucleic acids. I have an Associates of Applied Science in Biotechnology. I've transformed multiple microbes using various techniques and transfected live Mm5MT cells and NmuMG cells. I have experience amplifying nucleic acids using PCR and loop-mediated isothermal reactions.

Microbiology

I am currently an intern with the Texas Department of State Health Services Molecular Biology Lab. I have experience in molecular and conventional serotyping of Salmonella and Shigella. I understand the adaptive and innate immune system. I've developed and validated numerous SOPs for the State Molecular Lab including an intestinal parasite screen using real time PCR.

Physical Science

I have an Associates of Science in Biotechnology. I am a National Science Foundation scholarship award winner. I have a strong background in molecular biology and would like to help you meet your academic goals.

Austin Community College
Biotechnology

Education

Austin Community College (Biotechnology)

Hourly rate

Standard Hourly Rate: $42.00

Cancellation: 2 hours notice required

Travel policy

James will travel within 25 miles of Austin, TX 78754.